Anti-MIF Antibody [MIF/3489] - BSA and Azide free (A278295)

Name: Anti-MIF Antibody [MIF/3489] - BSA and Azide free
Brand: Antibodies.com
SKU: A278295
Price: 525.0 USD
Availability: InStock

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$525

Mouse monoclonal [MIF/3489] antibody to MIF for WB and IHC-P.

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Alternative Formats and ConjugatesAlternative Formats

Unconjugated

Delivery

Freight Charges

$40 to United States

Lead Time

4-6 Business Days

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Telephone

+1 (314) 370-6046

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Monospecific Antibody

Specifications

Name

Anti-MIF Antibody [MIF/3489] - BSA and Azide free

Description

Mouse monoclonal [MIF/3489] antibody to MIF.

Specificity

Macrophage migration inhibitory factor, known as MIF or glycosylationinhibiting factor, is a secreted, homotrimeric, pro-inflammatory cytokine that modulates macrophage and T cell function and is an important regulator of host response to infection. MIF is expressed at sites of inflammation, which suggests that it plays a role in regulating macrophage function in host defense. MIF is produced by the pituitary gland and is found in monocytes, macrophages, differentiating immunological cells in the eye lens and brain, and fibroblasts. Elevated levels of MIF protein are detected in the plasma of patients with severe sepsis or septic shock, a condition where MIF influences endotoxic shock by enhancing the production of other inflammatory cytokines including tumor necrosis factor Î± (TNFÎ±), interleukin-1 (IL-1) and interferon-Î³ (IFN-Î³). MIF promotes the systemic inflammatory response by counter-regulating glucocorticoid-mediated inhibition of immune-cell activation and proinflammatory cytokine production. MIF may mediate tissue destruction through the induction of proteinases.

Applications

WB, IHC-P

Dilutions

WB: 1-2 µg/ml, IHC-P: 1-2 µg/ml

Reactivity

Human

Immunogen

Recombinant fragment corresponding to human MIF protein. The exact sequence is proprietary.

Host

Mouse

Clonality

Monoclonal

Clone ID

MIF/3489

Isotype

IgG2a

Light Chains

kappa

Conjugate

Unconjugated

Purification

Protein A/G chromatography.

Concentration

1 mg/ml

Molecular Weight

13 kDa

Product Form

Liquid

Formulation

Supplied in 10mM Phosphate Buffered Saline; without Sodium Azide and carrier free.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

General Notes

This monoclonal antibody is also available in a different formulation with BSA and Sodium Azide - Anti-MIF Antibody [MIF/3489] (A277707).

Synonyms

GIF, GLIF, Glycosylation inhibiting factor, Glycosylation-inhibiting factor, L-dopachrome isomerase, L-dopachrome tautomerase, Macrophage migration inhibitory factor, Macrophage migration inhibitory factor (glycosylation-inhibiting factor), MIF protein, MIF_HUMAN, MMIF, Phenylpyruvate tautomerase

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Immunohistochemistry - Anti-MIF Antibody [MIF/3489] - BSA and Azide free (A278295)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human prostate tissue using Anti-MIF Antibody [MIF/3489]. Inset: PBS instead of the primary antibody. Secondary antibody negative control.

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Figure 2: Western Blot - Anti-MIF Antibody [MIF/3489] - BSA and Azide free (A278295)

Western blot analysis of (Lane 1) LNCaP cell line lysate and (Lane 2) PC3 cell line lysate using Anti-MIF Antibody [MIF/3489].

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Figure 3: Anti-MIF Antibody [MIF/3489] - BSA and Azide free (A278295)

Analysis of protein array containing more than 19,000 full-length human proteins using Anti-MIF Antibody [MIF/3489]. Z-Score and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt^TM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt^TM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target; a MAb is considered to be specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

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