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Anti-PCNA Antibody (A12584)

Western Blot - Anti-PCNA Antibody (A12584) - Antibodies.com Western Blot - Anti-PCNA Antibody (A12584) - Antibodies.com
$335
Product Datasheet

Rabbit polyclonal antibody to PCNA for WB.

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Anti-PCNA Antibody
Rabbit polyclonal antibody to PCNA for WB, IP and ChIP.
Name
Anti-PCNA Antibody
Description
Rabbit polyclonal antibody to PCNA.
Applications
WB
Dilutions
WB: 1:500-1:2,000
Reactivity
Human, Mouse, Rat, African green monkey
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 162-261 of human PCNA (NP_002583.1).
Sequence
CAKDGVKFSASGELGNGNIKLSQTSNVDKEEEAVTIEMNEPVQLTFALRYLNFFTKATPLSSTVTLSMSADVPLVVEYKIADMGHLKYYLAPKIEDEEGS
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
34 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
ATLD2, cb16, Cyclin, DNA polymerase delta auxiliary protein, etID36690.10, fa28e03, fb36g03, HGCN8729, MGC8367, Mutagen-sensitive 209 protein, OTTHUMP00000030189, OTTHUMP00000030190, Pcna/cyclin, PCNAR, PCNA_HUMAN, Polymerase delta accessory protein, Proliferating cell nuclear antigen, wu:fa28e03, wu:fb36g03
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-PCNA Antibody (A12584) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-PCNA Antibody (A12584)
Western blot analysis of extracts of various cell lines, using Anti-PCNA Antibody (A12584) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.
Western Blot - Anti-PCNA Antibody (A12584) - Antibodies.comEnlarge Image
Figure 2: Western Blot - Anti-PCNA Antibody (A12584)
Western blot analysis of extracts of various cell lines, using Anti-PCNA Antibody (A12584) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
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