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Anti-PCNA Antibody (A359)

Western Blot of PCNA in HeLa cells and Xenopus egg extract. Anti-PCNA Antibody was used at 1:1,000 dilution. Goat Anti-Rabbit IgG Antibody (HRP) was used as a secondary at 1:10,000 dilution. Lane 1: HeLa cell extract (20µg). Lane 2: Xenopus egg extract (20µg). Molecular mass of PCNA is 29 kDa. Western blot of PCNA in the whole cell extracts. Anti-PCNA Antibody was used at 1:1,000 dilution. Goat Anti-Rabbit IgG Antibody (HRP) was used as secondary at 1:10,000 dilution. Lane 1: Full-size recombinant human PCNA protein (5ng). Lane 2: HeLa (human) (20µg). Lane 3: MCF7 (human) (20µg). Lane 4: NIH3T3 (mouse) (20µg). Lane 5: CHO (hamster) (20µg). Lane 6: Xenopus laevis egg (frog) (20µg). Immunofluorescence staining of PCNA in GM0637 cells (human fibroblast ). Cells were fixed with 4% paraformaldehyde, permeabilized with 0.5% Triton and added with Anti-PCNA Antibody at 1:100 dilution. Goat Anti-Rabbit IgG Antibody (Alexa 488) was used as secondary at 1:10,000 dilution. DNA was stained with Heachst. PCNA foci were detected in the nuclei.
$390

Rabbit polyclonal antibody to PCNA for WB, IP, ICC/IF, DB and ELISA.

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Anti-PCNA Antibody
Rabbit polyclonal antibody to PCNA for WB, IP and ChIP.
Name
Anti-PCNA Antibody
Description
Rabbit polyclonal antibody to PCNA.
Applications
WB, IP, ICC/IF, DB, ELISA
Dilutions
WB: 1:1,000-1:2,000, IP: 1:100-1:500, ICC/IF: 1:100-1:1,000, DB: 1:500-1:2,000, ELISA: 1:500-1:2,000
Reactivity
Human, Mouse, Rat, Hamster, Chicken, Xenopus
Immunogen
Recombinant full length human PCNA protein.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 50% Glycerol (filter sterilised, without Sodium Azide, and carrier free).
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
ATLD2, cb16, Cyclin, DNA polymerase delta auxiliary protein, etID36690.10, fa28e03, fb36g03, HGCN8729, MGC8367, Mutagen-sensitive 209 protein, OTTHUMP00000030189, OTTHUMP00000030190, Pcna/cyclin, PCNAR, PCNA_HUMAN, Polymerase delta accessory protein, Proliferating cell nuclear antigen, wu:fa28e03, wu:fb36g03
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot of PCNA in HeLa cells and Xenopus egg extract. Anti-PCNA Antibody was used at 1:1,000 dilution. Goat Anti-Rabbit IgG Antibody (HRP) was used as a secondary at 1:10,000 dilution. Lane 1: HeLa cell extract (20µg). Lane 2: Xenopus egg extract (20µg). Molecular mass of PCNA is 29 kDa.Enlarge Image
Figure 1: Western Blot - Anti-PCNA Antibody (A359)
Anti-PCNA Antibody
Western blot of PCNA in the whole cell extracts. Anti-PCNA Antibody was used at 1:1,000 dilution. Goat Anti-Rabbit IgG Antibody (HRP) was used as secondary at 1:10,000 dilution. Lane 1: Full-size recombinant human PCNA protein (5ng). Lane 2: HeLa (human) (20µg). Lane 3: MCF7 (human) (20µg). Lane 4: NIH3T3 (mouse) (20µg). Lane 5: CHO (hamster) (20µg). Lane 6: Xenopus laevis egg (frog) (20µg).Enlarge Image
Figure 2: Anti-PCNA Antibody (A359)
Anti-PCNA Antibody
Immunofluorescence staining of PCNA in GM0637 cells (human fibroblast ). Cells were fixed with 4% paraformaldehyde, permeabilized with 0.5% Triton and added with Anti-PCNA Antibody at 1:100 dilution. Goat Anti-Rabbit IgG Antibody (Alexa 488) was used as secondary at 1:10,000 dilution. DNA was stained with Heachst. PCNA foci were detected in the nuclei.Enlarge Image
Figure 3: Immunofluorescence - Anti-PCNA Antibody (A359)
Anti-PCNA Antibody
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