Anti-STAT3 Antibody [ARC0045] (A306989)

Name: Anti-STAT3 Antibody [ARC0045]
Brand: Antibodies.com
SKU: A306989

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This product is discontinued

Anti-STAT3 Antibody [ARC0045] (A306989) has been discontinued and is no longer available.

View all Anti-STAT3 Antibodies.

Specifications

Name

Anti-STAT3 Antibody [ARC0045]

Description

Rabbit monoclonal [ARC0045] antibody to STAT3.

Applications

WB, IP

Dilutions

WB: 1:500-1:2,000, IP: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human STAT3 (P40763).

Sequence

MAQWNQLQQLDTRYLEQLHQLYSDSFPMELRQFLAPWIESQDWAYAASKESHATLVFHNLLGEIDQQYSRFLQESNVLYQHNLRRIKQFLQSRYLEKPME

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0045

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

1110034C02Rik, Acute Phase Response Factor, Acute-phase response factor, ADMIO, APRF, AW109958, DNA binding protein APRF, FLJ20882, HIES, MGC16063, Signal transducer and activator of transcription 3, Signal transducer and activator of transcription 3 (acute phase response factor), STAT 3, STAT3_HUMAN

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-STAT3 Antibody [ARC0045] (A306989)

Western blot analysis of extracts from wild type (WT) and STAT3 knockout (KO) HeLa cells, using Anti-STAT3 Antibody [ARC0045] (A306989) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1min.

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Figure 2: Western Blot - Anti-STAT3 Antibody [ARC0045] (A306989)

Western blot analysis of extracts of various cell lines, using Anti-STAT3 Antibody [ARC0045] (A306989) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1min.

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Figure 3: Western Blot - Anti-STAT3 Antibody [ARC0045] (A306989)

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Figure 4: Western Blot - Anti-STAT3 Antibody [ARC0045] (A306989)

Immunoprecipitation analysis of 300µg extracts of HeLa cells using 3µg of Anti-STAT3 Antibody [ARC0045] (A306989). This Western blot was performed on the immunoprecipitate using Anti-STAT3 Antibody [ARC0045] (A306989) at a dilution of 1:1000.

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