Anti-Vimentin Antibody [VM452] (A250307)

Mouse monoclonal [VM452] antibody to Vimentin for Flow Cytometry, WB and IHC-P.

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Email
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Name
Anti-Vimentin Antibody [VM452]
Description
Mouse monoclonal [VM452] antibody to Vimentin.
Specificity
This antibody reacts with a 58kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.
Applications
Flow Cytometry, WB, IHC-P
Dilutions
Flow Cytometry: 1-2 µg/million cells, WB: 1-2 µg/ml, IHC-P: 1-2 µg/ml
Reactivity
Human, Bovine, Canine, Feline, Porcine, Goat, Chicken
Cross Reactivity
This antibody does not cross react with Mouse or Rat.
Immunogen
Recombinant full-length human Vimentin protein.
Host
Mouse
Clonality
Monoclonal
Clone ID
VM452
Isotype
IgG1
Light Chains
kappa
Conjugate

Unconjugated

Purification
Protein A/G chromatography.
Concentration
200 µg/ml
Molecular Weight
57-60 kDa
Product Form
Liquid
Formulation
Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
General Notes
This monoclonal antibody is also available in a different formulation without BSA and Sodium Azide - Anti-Vimentin Antibody [VM452] - BSA and Azide free (A253487).
Synonyms
CTRCT30, Epididymis luminal protein 113, FLJ36605, HEL113, VIM, VIME_HUMAN
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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