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Primary Antibodies
GFP Antibodies
Anti-GFP Antibody [1A5] (A251)
Anti-GFP Antibody [1A5] (A251)
Overview
Specifications
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$435
Product Datasheet
Rat monoclonal (1A5) antibody to GFP for WB, IP, ICC, ChIP and ELISA.
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Product Size:
100µg
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Shipping Information
Freight/Packing Charges:
$40
Dispatched from St. Louis, MO.
Lead Time: 4-6 business days.
Specifications
Name
Anti-GFP Antibody [1A5]
Description
Rat monoclonal (1A5) antibody to GFP.
Specificity
This antibody reacts with GFP, EGFP, and their fused proteins.
Applications
WB
,
IP
,
ICC
,
ChIP
,
ELISA
Dilutions
WB: 1 µg/ml
Immunogen
Recombinant GFP protein.
Host
Rat
Clonality
Monoclonal
Clone ID
1A5
Isotype
IgG1
Conjugate
Unconjugated
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.2, with 50% Glycerol (filter sterilised, without Sodium Azide, and carrier free).
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Suitable Secondaries
Goat Anti-Rat IgG H&L Antibody (AP) (A294629)
Goat Anti-Rat IgG H&L Antibody (Biotin) (A294630)
Goat Anti-Rat IgG H&L Antibody (FITC) (A294635)
Goat Anti-Rat IgG H&L Antibody (HRP) (A294636)
See all Anti-Rat IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Scientific Validation Data
Validation Data
(3)
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Anti-GFP Antibody [1A5] (A251)
Anti-GFP Antibody
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Anti-GFP Antibody [1A5] (A251)
Anti-GFP Antibody
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Immunoprecipitation - Anti-GFP Antibody [1A5] (A251)
Anti-GFP Antibody
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Top
![Fluorescent image of COS1 cells due to GFP of GST-ZIPK fusion protein expressed in HEK293T cells (Right) and the same cells were immunostained using Anti-GFP Antibody [1A5], followed by Anti-Rat IgG (Texas Red) (Left). Note that fluorescence by the immunofluorescent staining using Anti-GFP Antibody [1A5] is much stronger than fluorescence due to GFP.](https://cdn.antibodies.com/image/catalog/0/A251_1.png?profile=product_top)
![Western blot analysis of GFP-ZIPK fusion protein over-expressed in HEK293 cells using Anti-GFP Antibody [1A5].](https://cdn.antibodies.com/image/catalog/0/A251_2.png?profile=product_top)
![Immunoprecipitation of GST-GFP fusion protein with Anti-GFP Antibody [1A5].](https://cdn.antibodies.com/image/catalog/0/A251_3.png?profile=product_top)
![Fluorescent image of COS1 cells due to GFP of GST-ZIPK fusion protein expressed in HEK293T cells (Right) and the same cells were immunostained using Anti-GFP Antibody [1A5], followed by Anti-Rat IgG (Texas Red) (Left). Note that fluorescence by the immunofluorescent staining using Anti-GFP Antibody [1A5] is much stronger than fluorescence due to GFP.](https://cdn.antibodies.com/image/catalog/0/A251_1.png?profile=product_top_thumb)
![Western blot analysis of GFP-ZIPK fusion protein over-expressed in HEK293 cells using Anti-GFP Antibody [1A5].](https://cdn.antibodies.com/image/catalog/0/A251_2.png?profile=product_top_thumb)
![Immunoprecipitation of GST-GFP fusion protein with Anti-GFP Antibody [1A5].](https://cdn.antibodies.com/image/catalog/0/A251_3.png?profile=product_top_thumb)
![Fluorescent image of COS1 cells due to GFP of GST-ZIPK fusion protein expressed in HEK293T cells (Right) and the same cells were immunostained using Anti-GFP Antibody [1A5], followed by Anti-Rat IgG (Texas Red) (Left). Note that fluorescence by the immunofluorescent staining using Anti-GFP Antibody [1A5] is much stronger than fluorescence due to GFP.](https://cdn.antibodies.com/image/catalog/0/A251_1.png?profile=product_image)
![Western blot analysis of GFP-ZIPK fusion protein over-expressed in HEK293 cells using Anti-GFP Antibody [1A5].](https://cdn.antibodies.com/image/catalog/0/A251_2.png?profile=product_image)
![Immunoprecipitation of GST-GFP fusion protein with Anti-GFP Antibody [1A5].](https://cdn.antibodies.com/image/catalog/0/A251_3.png?profile=product_image)
