Unconjugated
Studying biomolecules in their native environment represents the ideal sample condition for structural biology investigations. Here we present a novel protocol which allows to delivery proteins into eukaryotic cells through a mild thermal stimulation. The data presented herein show the efficacy of this approach for delivering proteins in the intracellular environment of mammalian cells reaching a concentration range suitable for successfully applying biophysical methods, such as double electron electron resonance (DEER) measurements for characterising protein conformations.
![Fluorescent image of COS1 cells due to GFP of GST-ZIPK fusion protein expressed in HEK293T cells (Right) and the same cells were immunostained using Anti-GFP Antibody [1A5], followed by Anti-Rat IgG (Texas Red) (Left). Note that fluorescence by the immunofluorescent staining using Anti-GFP Antibody [1A5] is much stronger than fluorescence due to GFP.](https://cdn.antibodies.com/image/catalog/0/A251_1.png?profile=product_alternative)
