Rabbit polyclonal antibody to Histone H4 (Acetyl-Lys5)
Specificity
The antibody detects endogenous level of Histone H4 only when acetylated at Lysine 5.
Applications
WB
Dilutions
Western blotting: 1:500~1:1000
Reactivity
Human, Mouse, Rat, Monkey
Immunogen
Peptide sequence around acetylation site of Lysine 5(S-G-R(p)-G-K) derived from Human Histone H4.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic acetylpeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific acetylpeptide. Non-acetyl specific antibodies were removed by chromatogramphy using non-acetylpeptide.
Concentration
1 mg/ml
Predicted MW
11kd
Formulation
Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.