Anti-GAPDH Anticorps [AMC0062R] (A17301)

Name: Anti-GAPDH Antibody [AMC0062R]
Brand: Antibodies.com
SKU: A17301

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Ce produit est discontinué

Anti-GAPDH Anticorps [AMC0062R] (A17301) a été abandonné et n'est plus disponible.

Voir toutes Anti-GAPDH Anticorps.

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Spécifications

Nom du produit

Anti-GAPDH Antibody [AMC0062R]

Description du produit

Mouse monoclonal [AMC0062R] antibody to GAPDH.

Applications

WB, IHC, ICC/IF

Recommander des dilutions

WB: 1:2,000-1:10,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivité

Human, Mouse, Rat

Immunogène

Recombinant fusion protein containing a sequence corresponding to amino acids 1-335 of human GAPDH (P04406).

Séquence

MGKVKVGVNGFGRIGRLVTRAAFNSGKVDIVAINDPFIDLNYMVYMFQYDSTHGKFHGTVKAENGKLVINGNPITIFQERDPSKIKWGDAGAEYVVESTGVFTTMEKAGAHLQGGAKRVIISAPSADAPMFVMGVNHEKYDNSLKIISNASCTTNCLAPLAKVIHDNFGIVEGLMTTVHAITATQKTVDGPSGKLWRDGRGALQNIIPASTGAAKAVGKVIPELNGKLTGMAFRVPTANVSVVDLTCRLEKPAKYDDIKKVVKQASEGPLKGILGYTEHQVVSSDFNSDTHSSTFDAGAGIALNDHFVKLISWYDNEFGYSNRVVDLMAHMASKE

Hôte

Mouse

Clonalité

Monoclonal

Clone

AMC0062R

Isotype

IgG

Conjuguer

Unconjugated

Purification

Affinity purification.

Masse moléculaire

36 kDa

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonymes

38 kDa BFA-dependent ADP-ribosylation substrate, aging associated gene 9 protein, Aging-associated gene 9 protein, BARS-38, cb609, EC 1.2.1.12, Epididymis secretory sperm binding protein Li 162eP, G3PD, G3PDH, G3P_HUMAN, GAPD, Glyceraldehyde 3 phosphate dehydrogenase, Glyceraldehyde-3-phosphate dehydrogenase, HEL S 162eP, HEL-S-162eP, KNC-NDS6, MGC102544, MGC102546, MGC103190, MGC103191, MGC105239, MGC127711, MGC88685, OCAS, p38 component, OCT1 coactivator in S phase, 38-KD component, peptidyl cysteine S nitrosylase GAPDH, Peptidyl-cysteine S-nitrosylase GAPDH, wu:fb33a10

Contrôles isotype

Mouse IgG (A17361)

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (4)

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Figure 1: Western Blot - Anti-GAPDH Antibody [AMC0062R] (A17301)

Western blot analysis of extracts of various cell lines, using Anti-GAPDH Antibody [AMC0062R] (A17301) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Immunohistochemistry - Anti-GAPDH Antibody [AMC0062R] (A17301)

Immunohistochemistry analysis of paraffin-embedded human kidney using Anti-GAPDH Antibody [AMC0062R] (A17301)Immunohistochemistry analysis of paraffin-embedded human kidney using GAPDH Mouse mAb (AC002) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-GAPDH Antibody [AMC0062R] (A17301)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-GAPDH Antibody [AMC0062R] (A17301)Immunohistochemistry analysis of paraffin-embedded mouse kidney using GAPDH Mouse mAb (AC002) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunofluorescence - Anti-GAPDH Antibody [AMC0062R] (A17301)

Immunofluorescence analysis of NIH/3T3 cells using Anti-GAPDH Antibody [AMC0062R] (A17301) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).