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Anti-His Tag Anticorps [AMC0149] (A17313)

Western Blot - Anti-His Tag Antibody [AMC0149] (A17313) - Antibodies.com Immunoprecipitation - Anti-His Tag Antibody [AMC0149] (A17313) - Antibodies.com Western Blot - Anti-His Tag Antibody [AMC0149] (A17313) - Antibodies.com
Recommandé
pour "His Tag Anticorps"
$230
Product Datasheet

Anticorps monoclonal [AMC0149] de souris contre His Tag pour WB et IP.

Qualité 100 % Garantie
Garantie de Correspond­ance des Prix

Frais de livraison
$40
Date de livraison
Livraison sous 5-8 jours ouvrables.

Téléphone
+1 (314) 370-6046
Lun - Ven, 8h - 16h AST
E-mail
orders@antibodies.com

Nom du produit
Anti-His Tag Antibody [AMC0149]
Description du produit
Mouse monoclonal [AMC0149] antibody to His Tag.
Applications
WB, IP
Recommander des dilutions
WB: 1:2,000-1:20,000, IP: 1:50-1:100
Reactivité
Species Independent
Immunogène
A synthetic peptide corresponding to His tag.
Séquence
HHHHHHH
Hôte
Mouse
Clonalité
Monoclonal
Clone
AMC0149
Isotype
IgG1
Conjuguer

Unconjugated

Purification
Affinity purification.
Masse moléculaire
27 kDa / 35 kDa
Forme du produit
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.
Stockage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonymes
6 His epitope tag, 6X His tag, Hexa His tag, HHHHHH epitope tag, HHHHHH tag, Polyhistidine Tag
Contrôles isotype
Anticorps secondaires appropriés
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Western Blot - Anti-His Tag Antibody [AMC0149] (A17313) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 1: Western Blot - Anti-His Tag Antibody [AMC0149] (A17313)
Western blot analysis of various lysates, using Anti-His Tag Antibody [AMC0149] (A17313) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Immunoprecipitation - Anti-His Tag Antibody [AMC0149] (A17313) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 2: Immunoprecipitation - Anti-His Tag Antibody [AMC0149] (A17313)
Immunoprecipitation of over-expressed His-tagged protein in 293T cells incubated using Anti-His Tag Antibody [AMC0149] (A17313). A mock served as negative control and over-expressed 293T cell lysate served as positive control.
Western Blot - Anti-His Tag Antibody [AMC0149] (A17313) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 3: Western Blot - Anti-His Tag Antibody [AMC0149] (A17313)
Immunoprecipitation analysis of 300µg extract cell lysate from 293T cells transfected with EPCAM expression vector containing a His-tag using 3µg of Anti-His Tag Antibody [AMC0149] (A17313). This Western blot was performed on the immunoprecipitate using Anti-His Tag Antibody [AMC0149] (A17313)at 1:5,000 dilution.

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