Anti-GAPDH Antibody [ARC50888] (A309068)

Anti-GAPDH Antibody [1D4]

A85382

229 Citations

Mouse monoclonal [1D4] antibody to GAPDH for WB, ICC/IF and IHC.

Anti-GAPDH Antibody [GA1R]

A85271

18 Citations

Mouse monoclonal (GA1R) antibody to GAPDH for Dot, ELISA, IS and WB.

(5)

A83722

Goat polyclonal antibody to GAPDH for ELISA, WB, IF and IHC.

(4)

A85377

Rabbit polyclonal antibody to GAPDH for WB, ICC/IF and IHC.

Anti-GAPDH Antibody [1D4]

A85382

229 Citations

Mouse monoclonal [1D4] antibody to GAPDH for WB, ICC/IF and IHC.

Anti-GAPDH Antibody [GA1R]

A85271

18 Citations

Mouse monoclonal (GA1R) antibody to GAPDH for Dot, ELISA, IS and WB.

(5)

A83722

Goat polyclonal antibody to GAPDH for ELISA, WB, IF and IHC.

(4)

A85377

See all Anti-Rabbit IgG Secondaries →

Rabbit polyclonal antibody to GAPDH for WB, ICC/IF and IHC.

Specifications

Name

Anti-GAPDH Antibody [ARC50888]

Description

Rabbit monoclonal [ARC50888] antibody to GAPDH.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:10,000-1:1:120,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 4-335 of human GAPDH (NP_002037.2).

Sequence

VKVGVNGFGRIGRLVTRAAFNSGKVDIVAINDPFIDLNYMVYMFQYDSTHGKFHGTVKAENGKLVINGNPITIFQERDPSKIKWGDAGAEYVVESTGVFTTMEKAGAHLQGGAKRVIISAPSADAPMFVMGVNHEKYDNSLKIISNASCTTNCLAPLAKVIHDNFGIVEGLMTTVHAITATQKTVDGPSGKLWRDGRGALQNIIPASTGAAKAVGKVIPELNGKLTGMAFRVPTANVSVVDLTCRLEKPAKYDDIKKVVKQASEGPLKGILGYTEHQVVSSDFNSDTHSSTFDAGAGIALNDHFVKLISWYDNEFGYSNRVVDLMAHMASKE

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC50888

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

36 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

38 kDa BFA-dependent ADP-ribosylation substrate, aging associated gene 9 protein, Aging-associated gene 9 protein, BARS-38, cb609, EC 1.2.1.12, Epididymis secretory sperm binding protein Li 162eP, G3PD, G3PDH, G3P_HUMAN, GAPD, Glyceraldehyde 3 phosphate dehydrogenase, Glyceraldehyde-3-phosphate dehydrogenase, HEL S 162eP, HEL-S-162eP, KNC-NDS6, MGC102544, MGC102546, MGC103190, MGC103191, MGC105239, MGC127711, MGC88685, OCAS, p38 component, OCT1 coactivator in S phase, 38-KD component, peptidyl cysteine S nitrosylase GAPDH, Peptidyl-cysteine S-nitrosylase GAPDH, wu:fb33a10

Isotype Controls

Suitable Secondaries

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (12)

Figure 1: Western Blot - Anti-GAPDH Antibody [ARC50888] (A309068)

Western blot analysis of extracts of HeLa cells, using Anti-GAPDH Antibody [ARC50888] (A309068) at 1:10,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

Figure 2: Western Blot - Anti-GAPDH Antibody [ARC50888] (A309068)

Western blot analysis of various lysates, using Anti-GAPDH Antibody [ARC50888] (A309068) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

Figure 3: Immunohistochemistry - Anti-GAPDH Antibody [ARC50888] (A309068)

Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using Anti-GAPDH Antibody [ARC50888] (A309068) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Figure 4: Immunohistochemistry - Anti-GAPDH Antibody [ARC50888] (A309068)

Immunohistochemistry analysis of paraffin-embedded human liver cancer using Anti-GAPDH Antibody [ARC50888] (A309068) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Figure 5: Immunohistochemistry - Anti-GAPDH Antibody [ARC50888] (A309068)

Immunohistochemistry analysis of paraffin-embedded human liver using Anti-GAPDH Antibody [ARC50888] (A309068) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Figure 6: Immunohistochemistry - Anti-GAPDH Antibody [ARC50888] (A309068)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-GAPDH Antibody [ARC50888] (A309068) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Figure 7: Immunohistochemistry - Anti-GAPDH Antibody [ARC50888] (A309068)

Immunohistochemistry analysis of paraffin-embedded mouse stomach using Anti-GAPDH Antibody [ARC50888] (A309068) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Figure 8: Immunohistochemistry - Anti-GAPDH Antibody [ARC50888] (A309068)

Immunohistochemistry analysis of paraffin-embedded rat kidney using Anti-GAPDH Antibody [ARC50888] (A309068) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Figure 9: Immunohistochemistry - Anti-GAPDH Antibody [ARC50888] (A309068)

Immunohistochemistry analysis of paraffin-embedded rat ovary using Anti-GAPDH Antibody [ARC50888] (A309068) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Figure 10: Immunofluorescence - Anti-GAPDH Antibody [ARC50888] (A309068)

Immunofluorescence analysis of NIH/3T3 using Anti-GAPDH Antibody [ARC50888] (A309068) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

Figure 11: Immunofluorescence - Anti-GAPDH Antibody [ARC50888] (A309068)

Immunofluorescence analysis of PC-12 using Anti-GAPDH Antibody [ARC50888] (A309068) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).