Anti-GAPDH Antibody (A85377)

Cytoplasm Marker Cytoskeleton Marker Loading Control Nuclear Marker

$185

Rabbit polyclonal antibody to GAPDH for WB, ICC/IF and IHC.

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Freight Charges

$40 to United States

Lead Time

4-6 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-GAPDH Antibody

Description

Rabbit polyclonal antibody to GAPDH.

Applications

WB, ICC/IF, IHC

Dilutions

WB: 1:30,000, ICC/IF: 1:2,000, IHC: 1:2,000

Reactivity

Human, Horse, Bovine, Porcine, Chicken, Rat, Mouse

Immunogen

Recombinant full-length human GAPDH, expressed in and purified from E. coli.

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Molecular Weight

36 kDa

Purity

Whole antiserum.

Product Form

Liquid

Formulation

Supplied as an aliquot of serum with 5mM Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

38 kDa BFA-dependent ADP-ribosylation substrate, aging associated gene 9 protein, Aging-associated gene 9 protein, BARS-38, cb609, EC 1.2.1.12, Epididymis secretory sperm binding protein Li 162eP, G3PD, G3PDH, G3P_HUMAN, GAPD, Glyceraldehyde 3 phosphate dehydrogenase, Glyceraldehyde-3-phosphate dehydrogenase, HEL S 162eP, HEL-S-162eP, KNC-NDS6, MGC102544, MGC102546, MGC103190, MGC103191, MGC105239, MGC127711, MGC88685, OCAS, p38 component, OCT1 coactivator in S phase, 38-KD component, peptidyl cysteine S nitrosylase GAPDH, Peptidyl-cysteine S-nitrosylase GAPDH, wu:fb33a10

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

Figure 1: Immunofluorescence - Anti-GAPDH Antibody (A85377)

HeLa cells stained with Anti-GAPDH Antibody (red), Anti-TAF15 Antibody (A85450 | green) and DNA (blue). The Anti-GAPDH Antibody reveals strong cytoplasmic staining, while the Anti-TAF15 Antibody reveals a granular nuclear localization.

Figure 2: Immunofluorescence - Anti-GAPDH Antibody (A85377)

Immunofluorescent analysis of HeLa cells stained with Anti-GAPDH Antibody (A85377), at a dilution of 1:2,000 in red. Blue is Hoechst staining of nuclear DNA. The Anti-GAPDH Antibody (A85377) produces diffuse cytoplasmic staining of cells.

Figure 3: Western Blot - Anti-GAPDH Antibody (A85377)

Western blot analysis of different cell cytosolic or nuclear enriched fractions, using Anti-GAPDH Antibody (A85377), at a dilution of 1:20,000, in red. The lanes contain samples of: [1] Protein standards, in red, [2] NIH-3T3 cytosolic, [3] NIH-3T3 nuclear, [4] HeLa cytosolic, and [5] HeLa nuclear fractions. Strong band at 37kDa corresponds to GAPDH protein, mainly detected in the cytosolic fractions. The same blot was simultaneously probed with Anti-SF3B4 Antibody (A85417), at a dilution of 1:1,000, in green. In contrast to GAPDH, SF3B4 is exclusively expressed in the nuclear fraction.

Figure 4: Western Blot - Anti-GAPDH Antibody (A85377)

Blot of HeLa cells probed with Anti-GAPDH Antibody. Note the single clean band at 36 kDa corresponding to GAPDH.

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