Recombinant rabbit monoclonal [DM1] antibody to His Tag.
Applications
ELISA, WB
Dilutions
WB: 1:1,000
Host
Rabbit
Clonality
Monoclonal
Clone ID
DM1
Isotype
IgG
Conjugate
Unconjugated
Purification
Affinity chromatography.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze / thaw cycles.
Synonyms
6 His epitope tag, 6X His tag, Hexa His tag, HHHHHH epitope tag, HHHHHH tag, Polyhistidine Tag
Figure 1: Western Blot - Anti-His Tag Antibody [DM1] - Azide free (A317439)
Western blot analysis of His-Tag fusion protein using Anti-His Tag Antibody [DM1] - Azide free (A317439) at 1:1,000 dilution. Secondary Antibody: Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:5,000 dilution. His-tag fusion protein: 70kDa, 20ng per lane. Blocking buffer: 5% non-fat dry milk in TBST. Exposure time: 1s.
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