The Notch signalling pathway is a highly conserved pathway that plays crucial roles in regulating various types of stem cells throughout development, in promoting tissue homeostasis, and in some disease states. In molecular terms, the Notch signalling pathway operates through a series of receptor-ligand interactions and downstream events. Key components of the pathway include Notch receptors (Notch1-4) and their ligands (Delta-like [DLL1, DLL3, DLL4] and Jagged [JAG1, JAG2]), which are transmembrane proteins. When a Notch receptor on one cell interacts with its ligand on an adjacent cell, it triggers proteolytic cleavage events that release the Notch intracellular domain (NICD). NICD then translocates to the nucleus and forms a complex with various transcriptional co-activators, leading to the activation of Notch target genes. Notch signalling thereby influences stem cell fate decisions by regulating the expression of genes that are involved in cell cycle progression, differentiation, or self-renewal. Notch often acts in conjunction with other signalling pathways, such as Wnt and BMP, to regulate stem cell behaviours. In early embryogenesis, Notch signalling is intimately involved in the formation of various tissue lineages. It regulates the differentiation of ESCs by influencing their commitment to specific cell fates. Notch signalling acts to establish boundaries between different cell populations, ensuring proper tissue patterning during development. Notch signalling has been implicated in the reprogramming of somatic cells into iPSCs. Activation of the Notch pathway enhances the efficiency of iPSC generation. It promotes the maintenance of pluripotency and inhibits the differentiation of reprogrammed cells, facilitating the successful generation of iPSCs. Notch signalling plays important additional roles in regulating tissue-specific or adult stem cells in various organs and tissues. For example, in the hematopoietic system, Notch signalling maintains the balance between self-renewal and differentiation of hematopoietic stem cells (HSCs). In the intestine, it is similarly essential for the regulation of intestinal stem cell (ISC) proliferation and differentiation. Notch signalling also influences the behaviour of neural stem cells (NSCs) in the brain, determining whether they remain quiescent or undergo proliferation and differentiation. Dysregulated Notch signalling has been associated with the development and maintenance of cancer stem cells (CSCs) in various malignancies, including breast cancer, leukaemia, and brain tumours. In CSCs, aberrant Notch activation can lead to uncontrolled self-renewal and subsequent resistance to chemotherapy or targeted therapies, contributing to tumour growth and recurrence. Thus, the Notch signalling pathway is a central regulator of stem cell biology, impacting various stem cell types across different tissues and developmental stages. Its role in promoting stem cell maintenance, regulating differentiation, and contributing to disease pathogenesis highlights its importance in both normal physiology and pathology. We offer a large product range of research tools for studying the Notch signalling pathway, including GSK3 beta antibodies, Notch1 antibodies, ADAM17 antibodies, NOTCH3 antibodies, and Jagged1 antibodies. Explore our full Notch signalling pathway product range below and discover more, for less. Alternatively, you can explore our Cytoplasmic, Surface Molecules, and Nuclear product ranges.