Anti-Histone H4 (acetyl Lys 12) Antibody (A320143)

Anti-Histone H4 (acetyl Lys 12) Antibody

Rabbit polyclonal antibody to Histone H4 (acetyl Lys 12).

This antibody recognises Histone H4 when acetylated at lysine 12.Histone H4 is one of the four core histones that make up the nucleosome core particle. Nucleosomes are the smallest subunit of chromatin and are made up of 146 bp of DNA wrapped around an octamer comprised of pairs of the four core histones (H2A, H2B, H3, and H4) (Smith, 1991). Histones may be modified in several ways including acetylation, methylation, phosphorylation, ubiquitination, glycosylation, and SUMOylation. Lysine residues within the N-terminal tail protruding from the histone core of the nucleosome are acetylated and deacetylated as part of gene regulation. Hyperacetylation of histone tails makes chromatin more accessible to DNA-binding proteins by weakening histone-DNA and nucleosome-nucleosome interactions. Acetylation of histone H4 usually results in transcriptional activation. Wide species cross-reactivity is expected from Rabbit anti Human histone H4 (Ac12) antibody based on sequence.

ChIP, IF, WB

ChIP: 10 µg per ChIP, IF: 1 µg/ml - 2 µg/ml, WB: 1 µg/ml - 2 µg/ml

Human, Mouse, Yeast, Broad

A peptide including acetylated lysine 12 of histone H4.

Rabbit

Polyclonal

IgG

Liquid

Supplied in Phosphate Buffered Saline with 0.035% Sodium Azide and 30% Glycerol.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Rabbit anti Human histone H4 (Ac12) antibody recognizes histone H4 when acetylated at lysine 12.Histone H4 is one of the four core histones that make up the nucleosome core particle. Nucleosomes are the smallest subunit of chromatin and are made up of 146 bp of DNA wrapped around an octamer comprised of pairs of the four core histones (H2A, H2B, H3, and H4) (Smith, 1991). Histones may be modified in several ways including acetylation, methylation, phosphorylation, ubiquitination, glycosylation, and SUMOylation (Koch et al. 2007). Lysineresidues within theN-terminaltail protruding from thehistonecore of thenucleosomeareacetylatedand deacetylated as part ofgene regulation. Hyperacetylation of histone tails makes chromatin more accessible toDNA-binding proteinsby weakening histone-DNA and nucleosome-nucleosome interactions (Kuo and Allis, 1998). Acetylation of histone H4 usually results in transcriptional activation (Turner et al. 1989).Wide species cross-reactivity is expected from Rabbit anti Human histone H4 (Ac12) antibody based on sequence.

H4/A, H4C1, H4FA, HIST1H4A