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Anti-AKT1 (phospho Thr450) Anticorps (A16395)

Western Blot - Anti-AKT1 (phospho Thr450) Antibody (A16395) - Antibodies.com
$435
Product Datasheet

Anticorps polyclonal de lapin contre AKT1 (phospho Thr450) pour WB.

Qualité 100 % Garantie
Garantie de Correspond­ance des Prix

Frais de livraison
$40
Date de livraison
Livraison sous 5-8 jours ouvrables.

Téléphone
+1 (314) 370-6046
Lun - Ven, 8h - 16h AST
E-mail
orders@antibodies.com

Nom du produit
Anti-AKT1 (phospho Thr450) Antibody
Description du produit
Rabbit polyclonal antibody to AKT1 (phospho Thr450).
Applications
WB
Recommander des dilutions
WB: 1:500-1:1,000
Reactivité
Human
Immunogène
A synthetic phosphorylated peptide around T450 of human Akt1 (NP_005154.2).
Séquence
TITPP
Hôte
Rabbit
Clonalité
Polyclonal
Isotype
IgG
Conjuguer

Unconjugated

Purification
Affinity purification.
Masse moléculaire
60 kDa
Forme du produit
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Stockage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonymes
AKT, AKT 1, AKT1_HUMAN, MGC99656, PKB, PKB-ALPHA, PRKBA, Protein kinase B, Protein Kinase B Alpha, Proto-oncogene c-Akt, RAC, RAC Alpha, RAC-alpha serine/threonine-protein kinase, RAC-PK-alpha
Contrôles isotype
Anticorps secondaires appropriés
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Western Blot - Anti-AKT1 (phospho Thr450) Antibody (A16395) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 1: Western Blot - Anti-AKT1 (phospho Thr450) Antibody (A16395)
Western blot analysis of extracts of 293 and HeLa cells, using Anti-AKT1 (phospho Thr450) Antibody (A16395) at 1:1,000 dilution. 293 cells were treated by Insulin (100nM) for 10 minutes after serum-starvation overnight. HeLa cells were treated by EGF (100ng/ml) for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 1min.

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