This antibody recognises glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a ~36 kDa multifunctional protein whose main function is to catalyse the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate, in conjunction with inorganic phosphate and nicotinamide adenine dinucleotide (NAD). This reaction is an important energy yielding step in carbohydrate metabolism. GAPDH has also been shown to translocate to the nucleus under a variety of stressors, most of which are associated with oxidative stress, whereby it mediates cell death. A further report has shown that GAPDH binds to several proteins that are responsible for neurodegenerative diseases, such as amyloid precursor protein and Huntingtin.
Applications
WB, ELISA, IF, IHC-Fr, IP
Reactivity
Rabbit, Human, Pig, Dog, Cat, Rat, Mouse, Xenopus, Tube-nosed Bat, Chicken, Vertebrates, Sheep, African green monkey , Crucian Carp
Immunogen
Rabbit muscle GAPDH.
Host
Mouse
Clonality
Monoclonal
Clone ID
6C5
Isotype
IgG1
Conjugate
Unconjugated
Purification
Protein A affinity chromatography of tissue culture supernatant.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with <0.1% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Mouse anti Rabbit GAPDH antibody, clone 6C5 recognizes glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a ~36 kDa multifunctional protein whose main function is to catalyse the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate, in conjunction with inorganic phosphate and nicotinamide adenine dinucleotide (NAD). This reaction is an important energy yielding step in carbohydrate metabolism. GAPDH has also been shown to translocate to the nucleus under a variety of stressors, most of which are associated with oxidative stress, whereby it mediates cell death. A further report has shown that GAPDH binds to several proteins that are responsible for neurodegenerative diseases, such as amyloid precursor protein and Huntingtin (Hara et al. 2006).
Western Blot - Anti-GAPDH Antibody [6C5] (A279474)
Western Blot analysis of GAPDH IP samples. IP was performed on Jurkat cell lysates using 10 µg (lanes 1 & 7), 5 µg (lanes 2 & 8), 1 µg (lanes 3 & 9) Anti-GAPDH Antibody [6C5] (A279474) and 10 µg Mouse IgG1 - Isotype Control (A283998). 8.75 µl of each IP was loaded onto an AnykD Criterion TGX Stain-Free gel. 8.75 µl Jurkat whole cell lysate (WCL) was run in lanes 5 & 11 while Precision Plus Protein Prestained Standards were run in lane 6. Rabbit anti GAPDH antibody was used at 1/1000 in lanes 1-5. HRP conjugated Tidyblot was used at 1/200 and visualized on the Chemidoc Touch Imaging System. Arrow points to GAPDH (molecular weight 37 kDa).
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