Anti-GAPDH Antibody (A320052)

Anti-GAPDH Antibody

Goat polyclonal antibody to GAPDH.

This antibody recognises an epitope within the C-terminal (CT) region of glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a ~36 kDa multifunctional protein whose main function is to catalyse the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate, in conjunction with inorganic phosphate and nicotinamide adenine dinucleotide (NAD). This reaction is an important energy yielding step in carbohydrate metabolism. GAPDH translocates to the nucleus under a variety of stressors, most of which are associated with oxidative stress, whereby it mediates cell death. GAPDH binds to several proteins that are responsible for neurodegenerative diseases, such as amyloid precursor protein and Huntingtin.

WB, IHC-P, ELISA

ELISA: 1:2,000, IHC-P: 0.3 µg/ml, WB: 0.01 - 0.03 µg/ml

Human, Dog, Mouse, Pig, Rat

A peptide sequence of C-HQVVSSDFNSDT corresponding to the C-terminus of GAPDH (NP_002037.2).

C-HQVVSSDFNSDT

Goat

Polyclonal

IgG

Approximately 35 kDa in human tonsil, mouse spleen and HeLa cell lysates.

Liquid

Supplied in TRIS Buffered Saline, 0.5% BSA and 0.02% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Goat anti Human GAPDH antibody recognizes an epitope within the C-terminal (CT) region of glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a ~36 kDa multifunctional protein whose main function is to catalyse the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate, in conjunction with inorganic phosphate and nicotinamide adenine dinucleotide (NAD). This reaction is an important energy yielding step in carbohydrate metabolism. GAPDH translocates to the nucleus under a variety of stressors, most of which are associated with oxidative stress, whereby it mediates cell death (Dastoor et al. 2001). GAPDH binds to several proteins that are responsible for neurodegenerative diseases, such as amyloid precursor protein and Huntingtin (Burke et al. 1996).

GAPD, Glyceraldehyde-3-phosphate dehydrogenase, Peptidyl-cysteine S-nitrosylase GAPDH