Anti-Histone H4 (acetyl Lys12) Antibody (A94792)

Name: Anti-Histone H4 (acetyl Lys12) Antibody
Brand: Antibodies.com
SKU: A94792
Price: 185.0 USD
Availability: InStock

This product is identical to Abcam (ab61238). Discover more for less.

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$185

Rabbit polyclonal antibody to Histone H4 (acetyl Lys12) for WB, IHC, IF and ELISA.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-Histone H4 (acetyl Lys12) Antibody

Description

Rabbit polyclonal antibody to Histone H4 (acetyl Lys12).

Specificity

This antibody detects endogenous levels of total Histone H4 protein only when acetylated at Lys12.

Applications

WB, IHC, IF, ELISA

Dilutions

IHC: 1:50-1:100, ELISA: 1:40000

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide derived from human Histone H4 around the acetylated site of Lys12 (amino acids 10-59).

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.

Molecular Weight

11kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

H4F2, H4FN, H4_HUMAN, HIST1H4, HIST2H4, Hist4h4, HISTH4H4, Histone H4, methyl histone H4, methylated histone H4

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-Histone H4 (acetyl Lys12) Antibody (A94792)

Western blot analysis of lysates from COS7 cells, treated with TSA 400nM 24h using Anti-Histone H4 (acetyl Lys12) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.

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Figure 2: Immunohistochemistry - Anti-Histone H4 (acetyl Lys12) Antibody (A94792)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Anti-Histone H4 (acetyl Lys12) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.

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Figure 3: Immunofluorescence - Anti-Histone H4 (acetyl Lys12) Antibody (A94792)

Immunofluorescence analysis of HeLa cells using Anti-Histone H4 (acetyl Lys12) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.