Anti-NF-L Antibody [6H112] (A270560)

Epitope map of novel DegenoTag™antibodies on human NF-L including Anti-NF-L Antibody [6H112] (A270560).

Immunofluorescent analysis of a rat frontal cortex section stained with Anti-NF-L Antibody [6H112] (A270560) at a dilution of 1:2,000 (green) and costained with Anti-NeuN Antibody (A85403) at a dilution of 1:2,000 (red). Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45µM, and free-floating sections were stained with the above antibodies. The Anti-NF-L Antibody [6H112] (A270560) labels the cell bodies and processes of pyramidal neurons, as well as dendrites and axons of other neuronal cells, while the FOX3/NeuN antibody selectively stains nuclei and cytoplasm of neuronal cells.

Immunohistochemistry analysis of a formalin fixed paraffin embedded human cerebellum section with Anti-NF-L Antibody [6H112] (A270560) at a dilution of 1:4,000 detected with DAB (brown) using the Vector Labs ImmPRESS method and reagents with citra buffer retrieval. Counterstained with Hematoxylin (blue). Anti-NF-L Antibody [6H112] (A270560) labels neuronal cells and their processes. Note: this antibody performs well in testing with both 4% PFA and standard NBF fixed tissues and is the recommended clone for use in NF-L immunostaining of human tissue.

Western blot analysis of spinal cord lysates from different species using Anti-NF-L Antibody [6H112] (A270560) at a dilution of 1:5,000 (green). The lanes contain: [Lane 1] protein standard (red), [Lane 2] rat, [Lane 3] mouse, [Lane 4] pig, and [Lane 5] cow spinal cord. Strong band at about 70kDa corresponds to the NF-L protein.