Anti-NF-L Antibody (A85451)

Western blot of whole rat brain homogenate stained with Anti-NF-L Antibody, at a dilution of 1:15,000). A prominent band running with an apparent SDS-PAGE molecular weight of ~68 kDa corresponds to rodent NF-L.

Immunofluorescent analysis of rat cerebellum section stained with Anti-NF-L Antibody (A85451), at a dilution of 1:5,000, in red, and co-stained with Anti-beta Synuclein Antibody [6A10] (A270558), at a dilution of 1:500, in green. The nuclear DNA is visualised in blue using Hoechst staining. Following transcardial perfusion of the rat with 4% paraformaldehyde, the brain was post-fixed for 24 hours, cut to 45 µm, and free-floating sections were stained with the above antibodies. The Anti-NF-L Antibody (A85451) labels dendrites and axons of neuronal cells, and the Anti-beta Synuclein Antibody [6A10] (A270558) detects protein that is concentrated in synaptic regions.

Immunofluorescent analysis of mouse striatum section stained using Anti-CNPase Antibody (A270543), at a dilution of 1:3,000, in green, and co-stained with Anti-NF-L Antibody (A85451), at a dilution of 1:5,000, in red. Nuclear DNA is visualised in blue using Hoechst staining. Following transcardial perfusion of the mouse with 4% paraformaldehyde, the brain was post-fixed for 24 hours, cut to 45 µm, and free-floating sections were stained with the above antibodies. Anti-CNPase Antibody (A270543) stains myelin sheath and the plasma membranes of oligodendrocytes; the myelin producing cells of the CNS. Anti-NF-L Antibody (A85451) labels axons of neuronal cells enclosed by the myelin.

Immunofluorescent analysis of rat cortex section stained with Anti-Ankyrin 3 Antibody [2A8], at a dilution of 1:2,000, in green, and co-stained with Anti-NF-L Antibody (A85451), at a dilution of 1:5,000, in red. Nuclear DNA is visualised in blue using Hoechst staining. Following transcardial perfusion with 4% paraformaldehyde, the brain was post-fixed for 24 hours, cut to 45 µm, and free-floating sections were stained using the above antibodies. Anti-Ankyrin 3 Antibody [2A8] stains the axonal initial segments, while Anti-NF-L Antibody (A85451) labels dendrites and axons of neuronal cells.

Immunohistochemistry analysis of a formalin fixed paraffin embedded human cerebellum section with Anti-NF-L Antibody (A85451) at a dilution of 1:5,000. Anti-NF-L Antibody (A85451) labels the perinkarya and dendrites of Purkinje cells and the projections of neuronal cells within the granular layer. Note: this antibody performs well in testing with both 4% PFA and standard NBF fixed rat, mouse and human tissues.

Immunohistochemistry analysis of a formalin fixed paraffin embedded human cerebellum section with Anti-NF-M Antibody (A85323) at a dilution of 1:1,000 detected with DAB (brown) using the Vector Labs ImmPRESS method and reagents with citra buffer retrieval. Counterstained with Hematoxylin (blue). Anti-NF-L Antibody (A85451) labels the perinkarya and dendrites of Purkinje cells and the projections of neuronal cells within the granular layer. Note: this antibody performs well in testing with both 4% PFA and standard NBF fixed rat, mouse, and human tissues.

Immunohistochemistry analysis of a formalin fixed paraffin embedded human cerebellum section with Anti-NF-M Antibody (A85323) at a dilution of 1:2,000 detected with DAB (brown) using the Vector Labs ImmPRESS method and reagents with citra buffer retrieval. Counterstained with Hematoxylin (blue). Anti-NF-L Antibody (A85451) labels the perinkarya and dendrites of Purkinje cells and the projections of neuronal cells within the granular layer. Note: this antibody performs well in testing with both 4% PFA and standard NBF fixed rat, mouse, and human tissues.