Anti-NF-L Antibody [DA2] (A85454)

Immunofluorescent analysis of rat frontal cortex section stained with Anti-NF-L Antibody [DA2] (A85454) at a dilution of 1:500 (red) and costained with Anti-GFAP Antibody (A85307) at a dilution of 1:5,000 (green). Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45µM, and free-floating sections were stained with above antibodies. This antibody labels cell bodies and processes of pyramidal neurons, as well as dendrites and axons of other neuronal cells, while the GFAP antibody stains the network of glial cells.

Immunohistochemistry analysis of a formalin fixed paraffin embedded human cerebellum section with Anti-NF-L Antibody [DA2] (A85454) at a dilution of 1:2,000 detected in DAB (brown) following the ImmPress method with citra buffer retrieval. Counterstained with Hematoxylin (blue). Anti-NF-L Antibody [DA2] (A85454) labels neuronal cells and their processes. Note: this antibody performs well in testing with both 4% PFA and standard NBF fixed tissues and is the recommended clone for use in NF-L immunostaining of rat tissue. Mouse and human tissue has also been validated in paraffin immuno-histochemistry with this antibody.

Immunohistochemistry analysis of a formalin fixed paraffin embedded human cerebellum section with Anti-NF-L Antibody [DA2] (A85454) at a dilution of 1:2,000 detected with DAB (brown) using the Vector Labs ImmPRESS method and reagents with Borg retrieval. Counterstained with Hematoxylin (blue). The Anti-NF-L Antibody [DA2] (A85454) strongly labels the perikarya and processes of Purkinje cells. Note: this antibody performs well in testing with both 4% PFA and NBF fixed rat tissues but requires high pH retrieval to stain long term NBF fixed human tissue effectively.