Anti-NF-L Antibody (A270580)

Immunostaining of a coronal section of the spinal cord of a rat given a midline C4 contusion injury three days previously. Sections were stained with Anti-NF-L Antibody (A270580) (red) and Anti-NF-L Antibody [6H63] (A333276) (green). Anti-NF-L Antibody [6H63] (A333276) stains prominent aggregates of material concentrated in the lateral funiculi and the dorsal columns but seen in lesser amounts throughout the section. These are degenerating and degenerated axons damaged by the C4 lesion. The Anti-NF-L Antibody antibody binds the C-terminal “tail” region of NF-L which is absent or destroyed during degeneration, so the positive profiles are largely negative for Anti-NF-L Antibody.

Immunostaining of a coronal section of the spinal cord of a rat given a midline C4 contusion injury three days previously. Sections were stained with Anti-NF-L Antibody (A270580) (red) and Anti-NF-L Antibody [1B11] (A104310) (green). Anti-NF-L Antibody [1B11] (A104310) stains prominent aggregates of material concentrated in the lateral funiculi and the dorsal columns but seen in lesser amounts throughout the section. These are degenerating and degenerated axons damaged by the C4 lesion. The Anti-NF-L Antibody antibody binds the C-terminal “tail” region of NF-L which is absent or destroyed during degeneration, so the positive profiles are largely negative for Anti-NF-L Antibody.

Immunohistochemistry analysis of a formalin fixed paraffin embedded human cerebellum section with Anti-NF-L Antibody (A270580) at a dilution of 1:5,000 detected with DAB (brown) using the Vector Labs ImmPRESS method and reagents with citra buffer retrieval. Counterstained with Hematoxylin (blue). Anti-NF-L Antibody (A270580) strongly labels the axons and dendrites of Purkinje cells and the projections of neuronal cells within the granular layer. Note: this antibody performs well in testing with both 4% PFA and standard NBF fixed rat, mouse, and human tissues.