Anti-NF-L Antibody [7D1] (A85453)

Embryonic rat cortical cells grown in tissue culture and stained with Anti-NF-L Antibody (green) and Anti-NF-H Antibody (A85337 | red). The perikaryal and dendritic neurofilaments in the Large cell in middle are stained with Anti-NF-L Antibody but not with Anti-NF-H Antibody. In contrast both antibodies stain axonal neurofilaments which therefore appear orange.

Immunofluorescent analysis of a section of mouse cerebellum stained with Anti-NF-L Antibody [7D1] (A85453), at a dilution of 1:5,000 in green, and co-stained with Anti-FOX2 Antibody (A104328), at a dilution of 1:2,000 in red. The nuclear DNA is visualised in blue using Hoechst staining. Following transcardial perfusion of the mouse with 4% paraformaldehyde, the brain was post-fixed for 24 hours, cut to 45 µm, and free-floating sections were stained with the above antibodies. Anti-NF-L Antibody [7D1] (A85453) labels dendrites and axons of neuronal cells predominantly axons in white matter and basket cell axons associated with Purkinje cells. The Anti-FOX2 Antibody (A104328) reveals protein expressed in a subset of neurons, including the Purkinje cells.

Western blot analysis of different tissue lysates using Anti-NF-L Antibody [7D1] (A85453), at a dilution of 1:5,000, in green. The lanes contain samples of: [Lane 1] Protein standards, in red, [Lane 2] rat brain, [Lane 3] rat spinal cord, [Lane 4] mouse brain, and [Lane 5] mouse spinal cord. The strong band at 68 kDa corresponds to the NF-L protein.

Strip blots of rat spinal cord homogenate probed with two subclones of Anti-NF-L Antibody. Both subclones, one of which became the definitive antibody, stain strongly and specifically a band at 68 kDa with essentially no background.